Biotin-Avidin Amplified Elisa for Quantitation of Human IgA
- 1 March 1985
- journal article
- research article
- Published by Taylor & Francis in Journal of Immunoassay
- Vol. 6 (1-2) , 67-77
- https://doi.org/10.1080/01971528508063021
Abstract
A biotin-avidin amplified enzyme-linked immunosorbent assay (ELISA) for the measurement of human IgA in serum, colostrum, gastric juice, and supernatants of in vitro mitogen-stimulated human peripheral blood mononuclear cells is described. The ELISA reproducibly detects as little as one nanogram of IgA in a fifty microliter sample (20 ng/ml).Keywords
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