Distribution of Sensitizing Antibody in Human Serum Proteins Fractionated by Electrophoresis-Convection

Open Access
Publisher Website
Google Scholar
Abstract
Summary and Conclusions: Three sera, prepared from individuals allergic toward cottonseed or ragweed pollen, have each been partitioned into eight fractions by electrophoresis-convection and into two other fractions by salting out with ammonium sulphate. After being assayed for N content and analyzed for electrophoretic composition, each preparation was repeatedly examined for reagins by both the neutralization and the serum-dilution procedures of passive transference. Activity was found to be concentrated in fractions Va, Vb and VI, which were composed of molecules immobilized by electrophoresis-convection at pH 6 or 5.5. These preparations were rich in β-globulin and also contained γ1-globulin. Analysis of the relationship between activity and electrophoretic composition of the 10 fractions supported the hypothesis proposed in our earlier report that β-globulins were chiefly responsible for reaginicity. Participation of γ-globulins could not, however, be directly excluded by our studies.