Polarised interleukin 8 secretion by HT 29/19A cells.
Open Access
- 1 March 1994
- Vol. 35 (3) , 338-342
- https://doi.org/10.1136/gut.35.3.338
Abstract
Interleukin 8 is a neutrophil chemotactic and stimulating cytokine induced by various inflammatory stimuli, including tumour necrosis factor, interleukin 1, and endotoxin. The ability of HT 29/19A enterocytes to synthesise interleukin 8 was studied. The results show that interleukin 1 is an important stimulus for interleukin 8 synthesis and secretion by HT 29/19A cells, being more potent than tumour necrosis factor. The tumour necrosis factor and interleukin 1 induced interleukin 8 secretion by HT 29/19A cells was seen to be polarised according to the direction of stimulation. These results support the concept that mucosal cells (enterocytes) may play an important part in initiating mucosal inflammation. Furthermore, it is proposed that HT 29/19A cells constitute a tool to study stimulus directed polarised cytokine secretion.Keywords
This publication has 34 references indexed in Scilit:
- Purification and amino acid sequencing of NAF, a novel neutrophil-activating factor produced by monocytesPublished by Elsevier ,2004
- Apical to basolateral surface area ratio and polarity of MDCK cells grown on different supportsExperimental Cell Research, 1992
- Influenza virus A infection induces interleukin‐8 gene expression in human airway epitheial cellsFEBS Letters, 1992
- Increased concentrations of interleukin 1 beta, interleukin-2, and soluble interleukin-2 receptors in endoscopical mucosal biopsy specimens with active inflammatory bowel disease.Gut, 1992
- Enhancement of Growth of Virulent Strains of Escherichia coli by Interleukin-1Science, 1991
- Tumor necrosis factor induces contraction of mesangial cells and alters their cytoskeletonsKidney International, 1990
- Novel neutrophil-stimulating peptidesImmunology Today, 1989
- The Neutrophil-Activating Protein (NAP-1) Is Also Chemotactic for T LymphocytesScience, 1989
- Overview of Epithelial PolarityAnnual Review of Physiology, 1989
- Structure determination of a human lymphocyte derived neutrophil activating peptide (LYNAP)Biochemical and Biophysical Research Communications, 1988