Abstract
Soybeans were grown in the greenhouse for 4-5 weeks and transferred to a series of control rooms where various combinations of temp. during the photoperiod and the dark period were applied for 5 days. Photoperiods of both 8 and 16 hrs. were used. Initiation of flower primordia was influenced to a much greater extent by variation in temp. during the dark period than by variation during the photoperiod. When the temp. during the photoperiod was constant, a 55[degree] temp. during the dark period limited the amt. of initiation that occurred. At 65[degree] initiation was much more extensive. This rise of 10[degree] in temp. during the dark period produced a greater difference in initiation than any other temp. variation of the same amt. Variation in temp. resulted in the formation of different numbers of nodes, but these differences were not great enough to account for the differences in floral initiation. Fresh and dry wts., stem lengths, and leaf areas, were also influenced by the different temp. treatments. The carbohydrate and N content of the plants was also influenced greatly by the different temp. treatments: as the temp. during the dark period was increased the carbohydrates decreased and the N fractions increased. These changes in carbohydrate and N metabolism could not be correlated with the morphological responses. None of the temp. combinations used in these expts. brought about the initiation of flower primordia when photoperiods of 16 hrs. were used.

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