Extraction of Endogenous Ethylene and Ethane from Leaves ofPhaseolus vulgarisL.

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Abstract
A procedure to recover small volumes of gases from plant tissues by vacuum extraction is described. Changes in the volume of the gases and the concentrations of ethylene and ethane recovered from Phaseolus leaves were observed when the evacuation conditions during extraction were altered. These changes were different in the primary leaves and in the second trifoliate leaves. Thus, estimates of internal ethylene and ethane concentrations appear to be influenced both by the morphology of the tissue and the evacuation conditions employed. There was a positive correlation between the concentrations of ethylene and ethane in the gaseous extracts. The internal ethylene concentrations in the primary, first trifoliate, and second trifoliate leaves, considered together, were correlated with their respective rates of ethylene emanation.