Estimation of Vi Antibody Employing Erythrocytes Treated with Purified Vi Antigen.

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Abstract
The prepn. of a sensitive and specific Vi agglutination reagent by sensitization of human type O erythrocytes with Vi antigen isolated from V form Escherichia coli 5396/38 is described. Vi antibody content of rabbit and human sera was detd. by this method and the conventional bacterial agglutination test. The hemagglutination test provided a more sensitive measure of Vi antibody by a factor of 3-to 6-fold. Greater variability in Vi titers was encountered in testing the sera of typhoid carriers: however, both methods were in good agreement in the detection of Vi antibody in these sera. The advantages inherent in the Vi hemagglutination procedure are discussed.