Detection of hemolytic Listeria monocytogenes by using DNA colony hybridization
- 1 September 1987
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 53 (9) , 2256-2259
- https://doi.org/10.1128/aem.53.9.2256-2259.1987
Abstract
A fragment of about 500 base pairs of the beta-hemolysin gene from Listeria monocytogenes was used to screen different bacterial strains by DNA colony hybridization. The cells in the colonies were lysed by microwaves in the presence of sodium hydroxide. Of 52 different strains of Listeria species screened, only the DNA from beta-hemolytic (CAMP-positive) strains of L. monocytogenes hybridized with this probe.This publication has 17 references indexed in Scilit:
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