Analytical Chemical Studies on Amino Sugars. I. New Color Reaction of Hexosamines using p-Nitrobenzaldehyde and Tetraethylammonium Hydroxide

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Abstract
It was found that glucosamine-HCl reacted with p-nitrobenzaldehyde in pyridinemethanol solution to yield Schiff base and gave a blue color by addition of tetraethylammonium hydroxide solution. This new color reaction is selective for 2-amino-2-deoxy sugars and α-amino carbonyl compounds, such as aminoacetaldehyde, δ-aminolevulinic acid and α-aminoacetophenone. The microdetection methods for 2-amino-2-deoxy sugars and their derivatives were proposed and the reaction mechanism was investigated.

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