An Oxidative Metabolite of Pyridoxine in Human Urine

Abstract
A fluorescent compound occurs in human urine after ingestion of pyridoxine (B6). Heating the urine with acid converts the compound to one fluorescing at maximum above pH of 8.6, and having a fluorescing intensity 25 X greater than the first compound. The new substance was isolated in crystalline form from urine heated with acid. It is an oxidation product, a lactone of 2-methyl-3-hydroxy-4-carboxy-5-hydroxymethyl pyridine.[long dash]H. M. Kaplan.

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