DEVELOPMENTAL CHANGES IN THE DEGRADATION OF THYROTROPHIN RELEASING HORMONE BY THE SERUM AND BRAIN TISSUES OF THE MALE RAT
- 1 August 1977
- journal article
- research article
- Published by Bioscientifica in Journal of Endocrinology
- Vol. 74 (2) , 339-340
- https://doi.org/10.1677/joe.0.0740339
Abstract
*Laboratoire de Médecine Expérimentale, UER Médecine Nord, Boulevard Pierre Dramard, 13326 Marseille Cedex 3, France and †Department of Obstetrics and Gynecology, Southwestern Medical School, 5323 Harry Hines Boulevard, Dallas, Texas 75235, U.S.A. (Received 15 March 1977) Thyrotrophin releasing hormone (TRH) is rapidly degraded when incubated at 37 °C with plasma (Redding & Schally, 1969) or brain homogenates (Bassiri & Utiger, 1974) from adult rats. However, immunoreactive TRH is stable in serum obtained from rats less than 2 weeks old (Oliver, Taurog & Porter, 1974). No loss of biological or immunological TRH activity occurs during incubation with serum from 4- or 16-day-old rats (Neary, Kieffer, Federico, Mover, Maloof & Soodak, 1976). In this report, we have determined the TRH degrading activity of brain homogenates and serum obtained from male rats at various stages of development after birth. Synthetic TRH (1 ng, Beckman Instruments, Inc.) diluted in 50 μl phosphate-buffered saline (0·01This publication has 0 references indexed in Scilit: