Dissection of distinct human immunoregulatory T-cell subsets by a monoclonal antibody recognizing a cell surface antigen with wide tissue distribution.
- 1 May 1981
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 78 (5) , 3160-3164
- https://doi.org/10.1073/pnas.78.5.3160
Abstract
A monoclonal antibody, PVR-11, was obtained after hybridization of X63Ag8.653 murine myeloma cells with spleen cells from a mouse immunized with human lymphocytes. It recognizes a 175,000- to 185,000-dalton surface antigen present on .apprxeq. 80% of normal human peripheral T lymphocytes, 50% of non-T non-B cells and < 10% of B cells as determined by complement-dependent microcytotoxicity. It is also present on varioue leukemia T cells, on some but not all T lymphoblastoid cell lines and on a small fraction of some B lymphoblastoid cell lines. Some B cell chronic lymphocytic leukemia cells also express the PVR-11 antigen. Functional analysis of normal human T lymphocytes demonstrated that the PVR-11-depleted T-cell subset contains the precursors of both cytotoxic and suppressor cells but lacks helper cells. Cytotoxic effector T cells express the PVR-11 antigen. Antigenic determinants with relatively wide tissue distribution apparently can dissect functionally distinct human immunoregulatory T-cell subsets.This publication has 33 references indexed in Scilit:
- Immunologic characterization of chronic lymphocytic leukemia cellsCancer, 1977
- Detection, isolation, and functional characterization of two human T-cell subclasses bearing unique differentiation antigens.The Journal of Experimental Medicine, 1977
- Characterization of a Subpopulation of Human T Lymphocytes Reactive with an Heteroantiserum to Human BrainThe Journal of Immunology, 1976
- Enumeration and isolation of human T and B lymphocytes by rosette formation with antibody-coated erythrocytes.1975
- Continuous cultures of fused cells secreting antibody of predefined specificityNature, 1975
- Human T-cell heterogeneity as delineated with a specific human thymus lymphocyte antiserum. In vitro effects on mitogen response mixed leukocyte culture, cell-mediated lymphocytotoxicity, and lymphokine production.Journal of Clinical Investigation, 1975
- Immunologic functions of isolated human lymphocyte subpopulations. III. Specific allogeneic lympholysis mediated by human T cells alone.1975
- Studies on the human T-lymphocyte population. II. The use of a T-cell specific antibody in the partial isolation and characterization of the human lymphocyte receptor for sheep red blood cells.1974
- Cell surface markers for human T and B lymphocytesEuropean Journal of Immunology, 1974
- Function and distribution pattern of human T lymphocytes. I. Production of anti-T lymphocyte specific sera as estimated by cytotoxicity and elimination of function of lymphocytes.1973