Time-Resolved Total Internal Reflection Fluorometry Study on Polarity at a Liquid/Liquid Interface
- 20 April 2001
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 73 (11) , 2421-2428
- https://doi.org/10.1021/ac001124x
Abstract
The polarity of a water/oil (oil: cyclohexane, carbon tetrachloride, toluene, chlorobenzene, o-dichlorobenzene, or 1,2-dichloroethane) interface was investigated by means of time-resolved total-internal-reflection (TIR) fluorescence spectroscopy of a polarity-sensitive probe: sulforhodamine B (SRB). In bulk solutions, the nonradiative decay rate constant of SRB increased with an increase in a solvent polarity parameter [ET(30)], and this relationship was used to estimate the polarities of water/oil interfaces. For the oil having a relatively low solvent polarity [ET(30) < 35 kcal/mol], the polarity of the water/oil interface agreed with that of the arithmetic average of the polarities of the two phases [ET(30)calc]. For water/o-dichlorobenzene and water/1,2-dichloroethane interfaces [ET(30) of the oil > 35 kcal/mol], on the other hand, the interfacial polarity determined by TIR spectroscopy was lower than the ET(30)calc. The results are discussed in terms of thickness/roughness of the water/oil interface.Keywords
This publication has 24 references indexed in Scilit:
- Complexation Kinetics of 8-Quinolinol Derivatives with Ni(II) and Zn(II) at the 1,2-Dichloroethane−Water Interface As Studied by Electrolyte Ascending Electrode PolarographyLangmuir, 1999
- Complexation Kinetics of 5-Alkyloxymethyl-8-quinolinols at Liquid−Liquid Interfaces As Studied by Dynamic Interfacial TensiometryLangmuir, 1998
- Structure, Dynamics, and Electronic Spectrum of N,N‘-Diethyl-p-nitroaniline at Water Interfaces. A Molecular Dynamics StudyThe Journal of Physical Chemistry B, 1998
- Electronic spectra of dipolar solutes at liquid/liquid interfaces: Effect of interface structure and polarityThe Journal of Chemical Physics, 1997
- Microenvironments of 8-anilino-1-naphthalenesulfonate at the heptane-water interface: time-resolved total internal reflection fluorescence spectroscopyChemical Physics Letters, 1997
- An attenuated total internal reflectance spectroscopy study of ET(30) at the free oil-water interfaceColloids and Surfaces A: Physicochemical and Engineering Aspects, 1995
- Interfacial Phenomena in Ion-Association Extraction Kinetics of Iron(II) with 1,10-PhenanthrolinesBulletin of the Chemical Society of Japan, 1990
- Electrodeposition of bismuth, lead, thallium, and copper at silver(111) from aqueous solutions. Studies by Auger spectroscopy and low-energy electron diffractionLangmuir, 1988
- A single spectroscopic probe for the determination of both the interfacial solvent properties and electrostatic surface potential of model lipid membranesFaraday Discussions of the Chemical Society, 1986
- Investigation of micelles, microemulsions, and phospholipid bilayers with the pyridinium-N-phenolbetaine ET(30), a polarity probe for aqueous interfacesThe Journal of Physical Chemistry, 1981