EUROPEAN BREWERY CONVENTION THE E.B.C. SYSTEM OF REFERENCE FOR BARLEY PROTEINS

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Abstract
Electrophoresis on agar gels followed by immunological testing with a standard serum is used to recognize and identify individual proteins derived from barley. To date, 17 protein constituents have been mapped in this manner, and a scheme of reference is suggested which will allow future identifications to be incorporated within the framework of the standards now proposed. The method which was used to prepare the immune serum (samples of which may be obtained from the Secretariat of the European Brewery Convention) is described, and instructions are given for extracting proteins from barley and for carrying out the technique of immuno-electrophoretic analysis.