Application of a flexible‐film isolator for rearing specific pathogen‐free chickens and investigating poultry pathogens

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Abstract
A commercially-available flexible-film isolator kit was adapted for rearing specific pathogen free poultry and for carrying out experiments with avian pathogens. By increasing the standard air hose diameter from 32 mm to 75 mm and incorporating large metal filter canisters in place of the standard plastic filter sleeves, air flow rates were increased up to 8-fold. These changes allowed a greater number of birds to be maintained for longer periods without the previous problems of condensation of water vapor on the inside surfaces of the isolator. Fiberglass mat filters were shown to be efficient in retaining Newcastle disease virus when challenged by aerosol produced experimentally. Cross-contamination by virus infections between adjacent isolators was prevented for at least 12 wk. The use of air-tight seals between the isolator canopy and structural components, air-tight feeder and light supports, an automatic watering system and facilities to improve portability are described. The adaptations resulted in an isolator which was efficient to use and maintain.

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