ANALYSIS OF SOUTHERN ONTARIO ACTINOBACILLUS-(HAEMOPHILUS)-PLEUROPNEUMONIAE ISOLATES BY RESTRICTION ENDONUCLEASE FINGERPRINTING
- 1 April 1990
- journal article
- research article
- Vol. 54 (2) , 244-250
Abstract
Isolates of Actinobacillus (Haemophilus) pleuropneumoniae were studied by restriction endonuclease fingerprinting (REF) analysis using the enzymes BamHIand HindIII. Restriction fragments were resolved by polyacrylamide gel electrophoresis and visualized by silver staining. Except for serotypes 1 and 9, reference strains of A. pleuropneumoniae serotypes 1 to 10 had clearly distinguishable REF profiles. Analysis of REF profiles of southern Ontario field isolates revealed limited heterogeneity amongst isolates of serotype 1 or serotype 5. The REF profiles of the serotype 7 isolates studied showed greater variation. Herterogeneity could not be correlated with the presence of plasmids nor with antibiotic resistance. Limited heterogeneity could also be detected amongst REF profiles of A. pleuropneumoniae isolates recovered from a closed herd suggesting that there is a small amount of genetic variation within clonal populations.This publication has 32 references indexed in Scilit:
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