Reduction of Ferric Phenanthroline—A Procedure for Determining Serum Uric Acid
- 1 November 1973
- journal article
- research article
- Published by Oxford University Press (OUP) in American Journal of Clinical Pathology
- Vol. 60 (5) , 691-694
- https://doi.org/10.1093/ajcp/60.5.691
Abstract
A method for the determination of uric acid with ferric phenanthroline in a maleic acid buffer at pH 5.5 is presented. The procedure yields accurate (r2 = 0.96; recovery 99.3%) and precise (C.V. = 2.08%) results in 5 minutes, usually without deproteinization. The method appears to be more specific for uric acid than the reduction of alkaline phosphotungstate and compares well with uricase determination. Elevated ascorbic acid above 3 mg. per 100 ml. interferes.Keywords
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