Lipid Metabolism of Developing Central Nervous Tissues in Organotypic Cultures

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Abstract
Organotypic cultures of rat cerebral cortex and cerebellum have been supplemented with different media. Fatty acid analysis in incubated media and in tissue explants revealed a characteristic differentiation of three periods during maturation: a post-traumatic repair phase, a synaptogenic period and a synaptic stage. Medium fatty acids are biphysically depleted from the nutrient. Both tissues differ in the depletion pattern of individual fatty acids. The development of tissue fatty acids is mainly observed during the synaptogenic period. Turnover studies of individual fatty acids indicate a tissue-specific and time-dependent characteristic.