Cell Wall Binding Properties of the Bacillus subtilis Autolysin(s)
- 1 August 1970
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 103 (2) , 488-93
- https://doi.org/10.1128/jb.103.2.488-493.1970
Abstract
Cell walls isolated from exponentially growing Bacillus subtilis have autolysin(s) attached to them. An autolysin can be released from the walls by incubation at 0 C with 3 m LiCl. The enzyme can reattach to walls when the salt concentration is reduced. The bound enzyme cannot be removed or destroyed by washing the walls with 8 m urea at 0 C. The binding of free enzyme to walls at 0 C can take place normally in the presence of 2 m urea.Keywords
This publication has 10 references indexed in Scilit:
- Autolysin(s) of Bacillus subtilis as Dechaining EnzymeJournal of Bacteriology, 1970
- Dynamic interactions between cell wall polymers, extracellular proteases and autolytic enzymesBiochemical and Biophysical Research Communications, 1970
- Increase in Lytic Activity in Competent Cells of Bacillus subtilis After Uptake of Deoxyribonucleic AcidJournal of Bacteriology, 1970
- Autolytic Enzyme System of Streptococcus faecalis V. Nature of the Autolysin-Cell Wall Complex and Its Relationship to Properties of the Autolytic Enzyme of Streptococcus faecalisJournal of Bacteriology, 1969
- Autolytic Enzyme Associated with Cell Walls of Bacillus subtilisJournal of Biological Chemistry, 1966
- Biochemical Aspects of Competence in the Bacillus subtilis Transformation SystemPublished by Elsevier ,1963
- BIOCHEMICAL ASPECTS OF COMPETENCE IN BACILLUS SUBTILIS TRANSFORMATION SYSTEM .2. AUTOLYTIC ENZYME ACTIVITY OF CELL WALLS1963