A disruption‐replacement approach for the targeted integration of foreign genes in Hansenula polymorpha
- 1 October 1995
- Vol. 11 (13) , 1241-1247
- https://doi.org/10.1002/yea.320111304
Abstract
A system has been developed which allows the selection of integrative transformants with replacement of the Hansenula polymorpha methanol oxidase gene (MOX) with expression cassettes carrying heterologous gene under the control of the MOX promoter. The system is convenient for comparison of the expression levels of different constructs integrated into the same locus of the H. polymorpha genome. This system was used to compare the secretion levels of human urinary plasminogen activator, the secretion of which was directed by different signal sequences.Keywords
This publication has 23 references indexed in Scilit:
- High-level expression of foreign genes in Hansenula polymorphaBiotechnology Advances, 1992
- Heterologous Gene Expression in Hansenula Polymorpha: Efficient Secretion of GlucoamylaseNature Biotechnology, 1991
- An effective transformation method for Hansenula polymorphaCurrent Genetics, 1990
- Transformation of methylotrophic yeast Hansenula polymorpha: Cloning and expression of genesJournal of Basic Microbiology, 1988
- Engineering of the hydrophobic segment of the signal sequence for efficient secretion of human lysozyme by SaccharomycescerevisiaeBiochemical and Biophysical Research Communications, 1987
- Transformation of the methylotrophic yeast Hansenula polymorpha by autonomous replication and integration vectorsMolecular Genetics and Genomics, 1986
- [12] One-step gene disruption in yeastPublished by Elsevier ,1983
- Transformation in yeast: Development of a hybrid cloning vector and isolation of the can1 geneGene, 1979
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977
- The fibrin plate method for estimating fibrinolytic activityArchives of Biochemistry and Biophysics, 1952