Differentiating Abnormal Cells in Valencia Orange Vesicles
- 1 January 1943
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 18 (2) , 73-75
- https://doi.org/10.3109/10520294309105793
Abstract
Metamorphosing cells developing thick cell walls in the juice vesicle of granulated Valencia oranges, which cannot be detected by ordinary microscopical examination, may be differentiated in an early stage for microchemical analysis in lignification studies. Thick sections of the juice vesicle are treated overnight in a 1:10,000 aqueous solution of ruthenium red, followed by 5-10 minutes in a 1:5,000 aqueous solution of methylene blue, and then differentiated in water.Keywords
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