Incorporation of [ 15 N]Ammonia by the Cellulolytic Ruminal Bacteria Fibrobacter succinogenes BL2, Ruminococcus albus SY3, and Ruminococcus flavefaciens 17
Open Access
- 1 June 2001
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 67 (6) , 2819-2822
- https://doi.org/10.1128/aem.67.6.2819-2822.2001
Abstract
The origin of cell nitrogen and amino acid nitrogen during growth of ruminal cellulolytic bacteria in different growth media was investigated by using 15 NH 3 . At high concentrations of peptides (Trypticase, 10 g/liter) and amino acids (15.5 g/liter), significant amounts of cell nitrogen of Fibrobacter succinogenes BL2 (51%), Ruminococcus flavefaciens 17 (43%), and Ruminococcus albus SY3 (46%) were derived from non-NH 3 -N. With peptides at 1 g/liter, a mean of 80% of cell nitrogen was from NH 3 . More cell nitrogen was formed from NH 3 during growth on cellobiose compared with growth on cellulose in all media. Phenylalanine was essential for F. succinogenes , and its 15 N enrichment declined more than that of other amino acids in all species when amino acids were added to the medium.Keywords
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