cDNA cloning and characterization of a novel gene encoding the MLF1-interacting protein MLF1IP
- 29 April 2004
- journal article
- Published by Springer Nature in Oncogene
- Vol. 23 (20) , 3700-3707
- https://doi.org/10.1038/sj.onc.1207448
Abstract
Myelodysplasia/acute myeloid leukemia (MDS/AML) is characterized by a t(3;5)(q25.1;q34) chromosomal translocation that forms a fusion gene between nucleophosmin (NPM) and MDS/myeloid leukemia factor 1 (MLF1). We identified a novel protein, MLF1-interacting protein (MLF1IP), that specifically associates with MLF1 by yeast two-hybrid analysis and in pulldown assays, and colocalizes with it in both the nuclei and cytoplasm of cells. The MLF1IP gene locus is at chromosome 4q35.1 and is composed of 14 exons spanning 75.8 kb of genomic DNA. The MLF1IP cDNA encodes a 46-kDa protein that contains two bipartite and two classical nuclear localization signals, two nuclear receptor-binding motifs (LXXLL), two leucine zippers, two PEST residues and several potential phosphorylation sites. MLF1IP transcripts are expressed in a variety of tissues (e.g. fetal liver, bone marrow, thymus and testis). MLF1IP appears to be a lineage-specific gene whose expression is confined exclusively to the CFU-E erythroid precursor cells, but not in mature erythrocytes. These observations, together with previous data demonstrating a role for MLF1 in suppressing red cell maturation, suggest a possible role for MLF1IP and MLF1 deregulation in the genesis of erythroleukemias.Keywords
This publication has 26 references indexed in Scilit:
- MADM, a Novel Adaptor Protein That Mediates Phosphorylation of the 14-3-3 Binding Site of Myeloid Leukemia Factor 1Published by Elsevier ,2002
- The Amino-terminal Domain of the Vacuolar Proton-translocating ATPase a Subunit Controls Targeting and in Vivo Dissociation, and the Carboxyl-terminal Domain Affects Coupling of Proton Transport and ATP HydrolysisJournal of Biological Chemistry, 2001
- A Potential Role for Human Cohesin in Mitotic Spindle Aster AssemblyJournal of Biological Chemistry, 2001
- Specific Phosphorylation of Nucleophosmin on Thr199 by Cyclin- dependent Kinase 2-Cyclin E and Its Role in Centrosome DuplicationJournal of Biological Chemistry, 2001
- cDNA Cloning, Expression Pattern, and Chromosomal Localization of Mlf1, Murine Homologue of a Gene Involved in Myelodysplasia and Acute Myeloid LeukemiaThe American Journal of Pathology, 1999
- Apoptosis induced by the myelodysplastic syndrome-associated NPM-MLF1 chimeric proteinOncogene, 1999
- 14-3-3 Proteins Interact with Specific MEK KinasesPublished by Elsevier ,1998
- cDNA Cloning, Tissue Distribution, and Chromosomal Localization of Myelodysplasia/Myeloid Leukemia Factor 2 (MLF2)Genomics, 1996
- Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferaseGene, 1988
- THE ROLE OF ERYTHROPOIETIN IN REGULATION OF POPULATION SIZE AND CELL CYCLING OF EARLY AND LATE ERYTHROID PRECURSORS IN MOUSE BONE MARROWCell Proliferation, 1977