INFLUENZA A VIRUS INTERACTION WITH MURINE LYMPHOCYTES .1. INFLUENCE OF INFLUENZA-VIRUS A-JAPAN 305 (H2N2) ON PATTERN OF MIGRATION OF RECIRCULATING LYMPHOCYTES

  • 1 January 1976
    • journal article
    • research article
    • Vol. 117  (3) , 852-858
Abstract
51Cr-labeled rat thoracic duct lymphocytes (TDL) were incubated with virus at 37.degree. C for 1 h and then infused i.v. into syngeneic recipients which were killed 1 h later. Virus-treated TDL accumulated in the liver, and their recovery in lymph nodes and spleen was severely reduced. Changes in lymphocytes induced by virus developed rapidly and were evident after incubation for only 15 min. UV-irradiated virus altered the pattern of lymphocyte localization, but attachment of heat-inactivated virus to lymphocytes in vitro had no effect on their distribution in vivo. Some virus-treated TDL, initially sequestered in the liver, apparently later recovered their ability to circulate normally. Recovery was not complete, and a population of cells failed to regain their ability to home into lymph nodes. Influenza virus apparently affected the homing properties of T [thymus-derived] and B [bone marrow-derived] cells. Aberrations in lymphocyte homing were probably mediated by the viral neuraminidase which induces changes in the cell membrane leading to their accumulation in the liver.