Transient interaction of cpSRP54 with elongating nascent chains of the chloroplast‐encoded D1 protein; ‘cpSRP54 caught in the act’
- 10 July 2002
- journal article
- Published by Wiley in FEBS Letters
- Vol. 524 (1-3) , 127-133
- https://doi.org/10.1016/s0014-5793(02)03016-8
Abstract
The signal recognition particle (SRP) in bacteria and endoplasmic reticulum is involved in co‐translational targeting. Plastids contain cpSRP54 and cpSRP43, unique to plants, but lack a SRP RNA molecule. A role for cpSRP in biogenesis of plastid‐encoded membrane proteins has not been firmly established yet. In this study, a transient interaction between cpSRP54 and elongating D1 protein was observed using a homologous chloroplast translation system. Using the novel approach of cross‐linking at different time points during elongation of full‐length D1 protein, we showed that cpSRP54 interacts strongly with the elongating nascent chain forming two distinct cross‐linked products. However, this interaction did not lead to an elongation arrest and cpSRP54 was released from the nascent chains, once they were longer than ∼14 kDa. Detailed mutant analysis showed that the cpSRP54 interaction occurred via the first transmembrane domain, which could be replaced by other hydrophobic domains of more than 10 amino acids.Keywords
This publication has 27 references indexed in Scilit:
- Post-translational protein translocation into thylakoids by the Sec and ΔpH-dependent pathwaysBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2001
- Distinct Albino3-dependent and -independent Pathways for Thylakoid Membrane Protein InsertionJournal of Biological Chemistry, 2001
- In Vitro Reconstitution of Insertion and Processing of Cytochrome f in a Homologous Chloroplast Translation SystemPublished by Elsevier ,2001
- Ribosome exchange revisited: a mechanism for translation-coupled ribosome detachment from the ER membraneTrends in Cell Biology, 2001
- Biogenesis of the Chloroplast-Encoded D1 Protein: Regulation of Translation Elongation, Insertion, and Assembly into Photosystem IIPlant Cell, 2000
- Arabidopsis Mutants Lacking the 43- and 54-Kilodalton Subunits of the Chloroplast Signal Recognition Particle Have Distinct PhenotypesPlant Physiology, 1999
- A Chromodomain Protein Encoded by the Arabidopsis CAO Gene Is a Plant-Specific Component of the Chloroplast Signal Recognition Particle Pathway That Is Involved in LHCP TargetingPlant Cell, 1999
- Expression of a dominant negative form of cpSRP54 inhibits chloroplast biogenesis in ArabidopsisThe Plant Journal, 1998
- Kinetic Resolution of the Incorporation of the D1 Protein into Photosystem II and Localization of Assembly Intermediates in Thylakoid Membranes of Spinach ChloroplastsPublished by Elsevier ,1996
- A simple method for displaying the hydropathic character of a proteinJournal of Molecular Biology, 1982