Evidence of Zein-Bound Indoleacetic Acid Using Gas Chromatography-Selected Ion Monitoring-Mass Spectrometry Analysis and Immunogold Labeling
- 1 August 1991
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 96 (4) , 1070-1075
- https://doi.org/10.1104/pp.96.4.1070
Abstract
Commercial zein was base-hydrolyzed and purified extracts were subjected to gas chromatography-selected ion monitoring-mass spectrometry analysis. Indoleacetic acid (IAA) was shown to be released from this storage protein of corn (Zea mays). Isotope dilution using [13C6]IAA as an internal standard revealed a conservative ratio of 1 mole IAA to 175 moles zein. Immunoelectron micrographs of isolated protein bodies also showed IAA or an IAA-like molecule associated with zein and deposited within these organelles.Keywords
This publication has 10 references indexed in Scilit:
- Western Blot Analysis of Cereal Grain Prolamins Using an Antibody to Carboxyl-Linked Indoleacetic AcidPlant Physiology, 1991
- Exopolysaccharides Produced by Phytopathogenic Pseudomonas syringae Pathovars in Infected Leaves of Susceptible HostsPlant Physiology, 1989
- Isolation and Partial Characterization of the Major Amide-Linked Conjugate of Indole-3-Acetic Acid from Phaseolus vulgaris L.Plant Physiology, 1986
- 13C6-[Benzene Ring]-Indole-3-Acetic AcidPlant Physiology, 1986
- Synthesis and Deposition of Zein in Protein Bodies of Maize EndospermPlant Physiology, 1978
- Concentration of Indole-3-acetic Acid and Its Derivatives in PlantsPlant Physiology, 1977
- Auxin-binding Sites of Maize Coleoptiles Are Localized on Membranes of the Endoplasmic ReticulumPlant Physiology, 1977
- Esters of Indole-3-Acetic Acid from Avena SeedsPlant Physiology, 1976
- Zein synthesis in maize endosperm by polyribosomes attached to protein bodies.Proceedings of the National Academy of Sciences, 1976
- Purification and Partial Characterization of a Glucan Containing Indole-3-acetic AcidPlant Physiology, 1972