A simple method for measuring a picogram of acetylcholine using the clam (Mya arenaria) heart

Open Access

1 December 1970

journal article
Published by Wiley in British Journal of Pharmacology

Vol. 40 (4) , 866-870
https://doi.org/10.1111/j.1476-5381.1970.tb10661.x

Abstract

1 . A simple and reliable method is described for assaying 1 pg or less of acetylcholine (ACh) using a strip of clam (Mya arenaria) heart. 2 . Clam heart preparations generally are extremely selective for ACh. The preparation described is at least 10⁵ times less sensitive to the following: acetyl coenzyme A, adrenaline, choline, dopamine, γ-aminobutyric acid, glutamic acid, histamine, 5-hydroxytryptamine and noradrenaline. 3 . Because this preparation is one of the most sensitive known for detecting ACh, it should prove useful for assaying particularly low levels of this substance in biological extracts.

Keywords

This publication has 4 references indexed in Scilit:

Is the Mya arenaria heart suitable for ACh bioassay?
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