Enzymic synthesis of the trisaccharide core region of the carbohydrate chain ofN-glycoprotein
- 1 April 1994
- journal article
- Published by Springer Nature in Glycoconjugate Journal
- Vol. 11 (2) , 105-110
- https://doi.org/10.1007/bf00731150
Abstract
Transmannosylation from mannotriose (Manβ1-4Manβ1-4Man) to the 4-position at the nonreducing end N-acetylglucosaminyl residue ofN,N′-diacetylchitobiose was regioselectively induced through the use of β-d-mannanase fromAspergillus niger. The enzyme formed the trisaccharide Manβ1-4GlcNAcβ1-4GlcNAc (3.7% of the enzyme-catalysed net decrease ofN,N′-diacetylchitobiose) from mannotriose as a donor andN,N′-diacetylchitobiose as an acceptor. Mannobiose (Manβ1-4Man) was also shown to be useful as a donor substrate for the desired trisaccharide synthesis.Keywords
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