Autophagy of ferritin incorporated into the cytosol of HeLa cells by liposomes
- 1 February 1980
- journal article
- research article
- Published by Springer Nature in Cell and tissue research
- Vol. 205 (2) , 303-309
- https://doi.org/10.1007/bf00234688
Abstract
Summary Ferritin was trapped in negatively charged liposomes consisting of phosphatidyl choline and phosphatidyl serine in a molar ratio of 9∶1. Unilamellar vesicles were prepared by ultrasonic treatment. HeLa cells were incubated with these liposomes for 2 h and chased for 0, 2, 14 and 20 h. The migration pathway of ferritin in the cells was followed by quantitative electron microscopy. Ferritin was detected exclusively in cytosol and lysosomes. Evidence is presented that free ferritin in cytosol enters lysosomes.Keywords
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