GONADOTROPIC HORMONE: URINE ASSAYS OF NORMALLY CYCLING, MENOPAUSAL, CASTRATED, AND ESTRIN TREATED HUMAN FEMALES 1

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Abstract
Urines from 66 normal and menopausal women were concentrated and assayed for their gonadotropic potency. The conc. was effected by precipitating with 4 vol. of alc., collecting the precipitate at the centrifuge, washing with ether and dialysing out toxic salts against tap water. The potency was assayed by observing increase in wt. of immature rat uteri and ovaries when the concentrate was injected subcut. twice daily for 3 days. Their potency was not related to the presence or absence of symptoms, age, hysterectomy, or the involution. No difference in gonadotropic potency was found between menopausal women with symptoms and senile women, castrated women or menopausal women without symptoms. Urinary gonadotropic conc. was low in women with regular normal menstrual cycles, high in menopausal women in whom cycles had ceased, and intermediate in menopausal women with irregular cycles. Estrogen treatment alleviated the vasomotor symptoms of 15 menopausal women but failed to concurrently reduce their gonadotropic potency. Continued estrogen therapy caused a slight reduction in potency, but failed to suppress it by significant amts.

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