B lymphocytes contain three species of μ chains
- 1 December 1978
- journal article
- research article
- Published by Wiley in European Journal of Immunology
- Vol. 8 (12) , 876-880
- https://doi.org/10.1002/eji.1830081210
Abstract
The murine B cell line cloned from a single cell, 38C-13, synthesizes three species of μ chains, that of cell surface membrane IgM (m-μ), that of secreted IgM (s-μ) and that of intracellular IgM (i-μ). They differ in their mobility on sodium dodecyl sulfate polyacrylamide gel electrophoresis. Sequence analysis of the different μ chains suggests that they are identical in the N-terminal as well as in their C-terminal positions. The ratio between incorporated radioactive monosaccharides to radioactive amino acids into the three different μ chains was higher in s-μ than in m-μ, but nevertheless m-μ migrated more slowly than s-μ on sodium dodecyl sulfate polyacrylamide gel electrophoresis. However, since this ratio may also be influenced by the rate of synthesis, it may not represent a real molar ratio of carbohydrate to protein. Studies with normal spleen cells clearly indicated the presence of the same three types of μ chains.This publication has 32 references indexed in Scilit:
- Evidence for a C-terminal tyrosine residue in human and mouse B-lymphocyte membrane μ chainsNature, 1978
- Membrane immunoglobulins of bovine leukemic lymphocytesImmunochemistry, 1977
- Abnormalities in the glycosylation of immunoglobulin heavy chain and an H-2 transplantation antigen in a mouse myeloma mutantCell, 1977
- Immunoglobulin of T lymphoma cells is an integral membrane proteinNature, 1974
- Candidate for immunoglobulin D present on murine B lymphocytesNature, 1974
- CELL SURFACE IMMUNOGLOBULINThe Journal of Experimental Medicine, 1974
- THE K-CHAINS OF THE IMMUNOGLOBULIN FROM A CONTINUOUS CULTURE OF HUMAN LYMPHOCYTES (DAUDI) HAVE AN UNUSUAL MOLECULAR SIZEThe Journal of Experimental Medicine, 1974
- Difference in carbohydrate composition and a possible conformational difference between intracellular and extracellular immunoglobulin MBiochemistry, 1972
- Human erythrocyte membrane glycoprotein: A re-evaluation of the molecular weight as determined by SDS polyacrylamide gel electrophoresisBiochemical and Biophysical Research Communications, 1971
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970