Evaluation of a novel PCR-based diagnostic assay for detection of Mycobacterium tuberculosis in sputum samples
- 1 September 1996
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 34 (9) , 2307-2308
- https://doi.org/10.1128/jcm.34.9.2307-2308.1996
Abstract
We report on a PCR-based assay we have developed for the detection of Mycobacterium tuberculosis in sputum samples. One hundred sputum specimens, which included 34 culture-positive and 66 culture-negative specimens, were evaluated with this system. Of the 34 culture-positive specimens, 31 were PCR positive, and 60 of the culture-negative specimens were PCR negative. An internal standard has been included in the assay system to monitor PCR inhibition and to confirm the reliability of the PCR assay.Keywords
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