A lentiviral vector expressing a fusogenic glycoprotein for cancer gene therapy
- 1 October 2000
- journal article
- acquired diseases
- Published by Springer Nature in Gene Therapy
- Vol. 7 (19) , 1656-1663
- https://doi.org/10.1038/sj.gt.3301277
Abstract
The gibbon ape leukaemia virus envelope fusogenic membrane glycoprotein (GALV FMG) is a highly potent cytotoxic gene with great potential for use in cancer gene therapy. Here, we show that production of a VSV-G pseudotyped lentiviral vector expressing GALV FMG reconciles the requirements of viral production with the cytotoxic effects of GALV in human cells and has high titres on both dividing and quiescent tumour cells. Direct intratumoral injection of these stocks eradicated progressively growing human tumour xenografts. The potent bystander effect of the FMG transgene is a major contributor to the success of this approach but immunological activation may also be a factor. To our knowledge, this is the first demonstration in vivo of the potential both of FMG and lentiviral vectors for cancer gene therapy and highlights the importance of exploring different vector systems to complement the biological properties of the therapeutic transgene.Keywords
This publication has 36 references indexed in Scilit:
- A Hyperfusogenic Gibbon Ape Leukemia Envelope Glycoprotein: Targeting of a Cytotoxic Gene by Ligand DisplayHuman Gene Therapy, 2000
- Cancer gene therapy: hard lessons and new coursesGene Therapy, 2000
- Lentivirus-Mediated Transduction of Islet Grafts with Interleukin 4 Results in Sustained Gene Expression and Protection from InsulitisHuman Gene Therapy, 1998
- Recognition of Stress-Induced MHC Molecules by Intestinal Epithelial γδ T CellsScience, 1998
- Tumour cell expression of B7 costimulatory molecules and interleukin-12 or granulocyte–macrophage colony-stimulating factor induces a local antitumour response and may generate systemic protective immunityGene Therapy, 1998
- Lentiviral Vectors for Gene Therapy of Cystic FibrosisHuman Gene Therapy, 1997
- Multiply attenuated lentiviral vector achieves efficient gene delivery in vivoNature Biotechnology, 1997
- In Vivo Gene Delivery and Stable Transduction of Nondividing Cells by a Lentiviral VectorScience, 1996
- Paracrine Cytokine Adjuvants in Cancer ImmunotherapyAnnual Review of Immunology, 1995
- Construction and use of a safe and efficient amphotropic packaging cell lineVirology, 1988