Inhibition of Lymphocyte Stimulation by Cyclic and Non-Cyclic Nucleotides

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Abstract
Although little is known of the role of cyclic nucleotides in lymphocyte metabolism, Smith, Steiner and Parker have recently shown that exogenous cyclic and non-cyclic nucleotides inhibit the response of human lymphocytes to phytohemagglutinin (PHA). Furthermore, pharmacologic agents which raise the endogenous level of cyclic AMP (adenosine 3′,5′ monophosphoric acid) had the same effect (1). Studies in our laboratory confirm these observations with respect to PHA stimulation and extend them to the mixed lymphocyte reaction. Materials and Methods. Cell preparation. Sterile technique was employed. Normal human peripheral blood was drawn into a heparinized syringe and centrifuged at 4°C for 10 min at 120 × G. The supernatant plasma and the upper ⅕ of the red cells were drawn into a syringe and allowed to sediment for 30 min. The white cell-rich plasma was removed and centrifuged for 10 min at 200 × G.

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