Identification, purification and immunohistochemical detection of the inhibitor from porcine ovarian follicular fluid to compensatory ovarian hypertrophy in mice

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Abstract
Summary. An inhibitor to compensatory ovarian hypertrophy in mice was detected in porcine follicular fluid. Compensatory hypertrophy was inhibited by treatment with 0·6 ml whole follicular fluid, 0·2 ml of the non-dialysable fraction, or a fraction of the fluid salted out by ammonium sulphate at a saturation of 14·5–18·5%. The salted-out fraction was separable into two peaks by Sephadex G-200 column chromatography and the second peak, detectable as a single band by polyacrylamide gel disc electrophoresis, contained all the inhibitory activity. Specific fluorescence of an antiserum to the second peak was demonstrated at the granulosa cells.