EFFECT OF 5-HYDROXYTRYPTOPHAN ON PROLACTIN SECRETION IN THE MOUSE

Publisher Website
Google Scholar
Abstract
Division of Diabetes and Endocrinology, Scripps Clinic and Research Foundation, La Jolla, California 92037, U.S.A. (Received 9 February 1977) When administered systemically, 5-hydroxytryptophan increases the concentration of prolactin in the serum of rats (Lu & Meites, 1973) and man (Maclndoe & Turkington, 1973). Ergocornine administered to the mouse depresses the concentration of prolactin in both the pituitary gland and the serum (Sinha, Selby & VanderLaan, 1974). This study was designed to examine whether 5-hydroxytryptophan induces the secretion of prolactin in the mouse and whether ergocornine will inhibit the effects of 5-hydroxytryptophan. Male Swiss Webster mice aged 60-90 days and weighing 33-35 g were used. The mice were housed in plastic cages, five per cage. Blood specimens (100 μ) were collected without anaesthesia by orbital sinus puncture and the serum was separated and stored at −20°C for prolactin analysis by an homologous radioimmunoassay (Sinha, Selby, Lewis & VanderLaan, 1972). A 1:25 000

This publication has 0 references indexed in Scilit: