Enzyme-linked immunosorbent assay using solubilised antigen for detection of antibodies toAnaplasma marginale

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Abstract
Summary An enzyme-linked immunosorbent assay (ELISA) was used to detect antibodies againstAnaplasma marginale. A. marginale bodies were separated from parasitised erythrocytes by a modified nitrogen decompression method, sonicated, then solubilised with Triton X-100 and used as the ELISA antigen. In this ELISA system the required amount of antigen protein was 16.2 ng for each well. In the course of experimental infections, of calves, significant antibody levels were detected by ELISA and the complement fixation test (CFT) at almost the same time. Antibodies againstA. marginale were detectable for longer periods using the ELISA than using the CFT. Sera from calves infected withBabesia bigemina, B. bovis, B. ovata, Theileria sergenti andEperythrozoon wenyoni gave no reaction; however, antisera againstA. centrale did react with theA. marginale ELISA antigen.