COMPARISON OF CULTURED HUMAN MONONUCLEAR CELLS, LIMULUS AMEBOCYTE LYSATE and RABBITS IN THE DETECTION OF PYROGENS

28 June 2008

journal article
research article
Published by Hindawi Limited in Journal of Clinical Pharmacy & Therapeutics

Vol. 15 (6) , 425-433
https://doi.org/10.1111/j.1365-2710.1990.tb00407.x

Abstract

Isolated human mononuclear cells exposed to either lipopolysaccharide or Staphylococcus aureus secreted interleukin-1 like material. The secretion was concentration dependent. The minimal detectable level in the test solution of lipopolysaccharide and Staphylococcus aureus was 200 pg/ml and 10(5) cells/ml respectively. The sensitivity and specificity of the Limulus Amebocyte Lysate test, the rabbit pyrogen test and the monocyte test are compared. The monocyte test is proposed as an alternative in-vitro test to the rabbit pyrogen test.

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