Separation of megakaryocytes from rat bone marrow cells using velocity sedimentation in an isokinetic gradient of ficoll in tissue culture medium
- 1 July 1976
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 88 (3) , 317-322
- https://doi.org/10.1002/jcp.1040880307
Abstract
A technique for the purification of rat megakaryocytes is described. Velocity sedimentation in a previously described isokinetic gradient of Ficoll (polysucrose) in tissue culture medium was more effective than isopycnic sedimentation for the purification of megakaryocytes and resulted in preparations of megakaryocytes which contained 2.4 ± 0.8% (range 1.85–3.60%) megakaryocytes. Megakaryocytes exhibited a broad range of density between 1.06 and 1.15 gm/ml. The inaccuracy which is inherent in the use of velocity sedimentation without isopycnic sedimentation as a means of particle size analysis is discussed.This publication has 11 references indexed in Scilit:
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