The Importance of Escherichia coli Ribosomal Proteins L1, L11 and L16 for the Association of Ribosomal Subunits and the Formation of the 70‐S Initiation Complex
- 1 October 1975
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 58 (2) , 501-510
- https://doi.org/10.1111/j.1432-1033.1975.tb02398.x
Abstract
50-S subunits were washed with LiCl solutions of different concentrations. After washing with 1 M LiCl solution the particles lost their ability to attach either to 30-S subunits or to the AUG - 30-S subunit - fMet-tRNAfMet complex or to a poly(U) - 30-S subunit - Phe-tRNAPhe complex. Those proteins which were removed by LiCl were fractionated on a Sephadex G-100 column. Of the fractionated proteins only the combinations L1 and L11 or L1 and L16 were essential for the association of 50-S 1.0 cores (particles prepared by washing 50-S subunits in 1.0 M LiCl) with 30-S subunits. These three proteins were also required for the formation of a stable complex between 50-S 1.0 cores, mRNA, 30-S subunits and aminoacyl-tRNA.Keywords
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