Immunological screening method to detect specific translation products.

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Abstract
A very sensitive method to detect as antigens the presence of specific proteins within phage plaques or bacterial colonies is described. Plastic sheets are coated with antibody molecules, the sheet is exposed to lysed bacteria so that a released antigen can bind, and then the immobilized antigen is labeled with radioiodinated antibodies. Thus, the antigen is sandwiched between the antibodies attached to the plastic sheet and those carrying the radioactive label. Autoradiography then shows the positions of antigen-containing colonies or phage plaques. A few molecules of antigen released from each bacterial cell generate an adequate signal. [Escherichia coli and phage .lambda. were used.].