High Level of Expression of the Myelin Protein P0 in Chinese Hamster Ovary Cells
- 1 August 1990
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 55 (2) , 500-505
- https://doi.org/10.1111/j.1471-4159.1990.tb04163.x
Abstract
The major PNS myelin protein, P0, has been expressed in Chinese hamster ovary cells by transfection with a plasmid containing the P0-cDNA. The expression of P0 at both the RNA and the protein level was greatly increased, without detriment to the cell, by the dihydrofolate reductase/methotrexate strategy of gene amplication. The P0 expressed by these cells was glycosylated (containing approximately equal amounts of the complex and high-mannose type glycoproteins) and reached the plasma membrane. This system is suitable not only for addressing the function of P0 directly, but is also applicable to any protein of which an abundance is needed.This publication has 22 references indexed in Scilit:
- Expression and Localization of Myelin Basic Protein in Oligodendrocytes and Transfected FibroblastsJournal of Neurochemistry, 1988
- Primary Structure and Biochemical Properties of an M 2 Muscarinic ReceptorScience, 1987
- A sensitive immunotransfer method for the detection of oligodendroglial plasma membrane antigensNeurochemistry International, 1986
- Isolation and sequence of a cDNA encoding the major structural protein of peripheral myelinCell, 1985
- Expression of amplified human beta interferon genes using heavy metal induction in Chinese hamster ovary cellsGene, 1985
- Myelin-specific proteins and glycolipids in rat Schwann cells and oligodendrocytes in culture.The Journal of cell biology, 1980
- Compact myelin exists in the absence of basic protein in the shiverer mutant mouseNature, 1980
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970