SCANNING ELECTRON-MICROSCOPY OF CORNEAL WOUND-HEALING IN RABBIT

  • 1 January 1977
    • journal article
    • research article
    • Vol. 16  (9) , 787-796
Abstract
Corneal lesions 7.5 mm in diameter were made with an ocular trephine in rabbits. The time periods studied were 0, 30 min, and 1, 2, 4, 8, 16 and 24 h. At the end of the time period, the cornea was flooded with 4% glutaraldehyde, buffered with cacodylate, pH 7.4, and kept moist until removed. It was then fixed for 24 h. Half of the sample was dehydrated in graded alcohols, critical-point-dried, coated with Au-Pd alloy, and viewed in an AMR-1000 scanning electron microscope at an accelerating voltage of 20 kV. From 0-4 h cell trauma, debris and retraction were seen at the margin of the lesion. From 8-24 h a significant number of polymorphonuclear leukocytes were present over the total surface but in especially large numbers at the wound margin. At 16-24 h evidence of cell movement was present. Cells showed ruffling membranes, a decreased number of microvilli and a few filopodia along the advancing edge.

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