Improved measurement of13C,31P J coupling constants in isotopically labeled RNA
- 3 April 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 362 (2) , 156-160
- https://doi.org/10.1016/0014-5793(95)00232-x
Abstract
3JCPcoupling constants have been measured in 1 99%13C,15N labeled lead‐dependent ribozyme, known as the leadzyme. These coupling constants were determined by analysis of the intensity of individual crosspeaks in a spin‐echo difference constant time HSQC experiment. This procedure permits improved measurement of the3JC2′Pand3JC4′Pcoupling constants in isotopically labeled RNA and yielded valuable information on the β and ϵ backbone torsion angles in the leadzyme.Keywords
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