Synthesis and properties of human γ‐lipotropin

Abstract
The synthesis of human .gamma.-lipotropin by the solid-phase method is described. The synthetic product was characterized by its migration factor in partition chromatography on Sephadex G-50, paper electrophoresis, polyacrylamide gel electrohoresis, TLC, high performance liquid chromatography, end group determination, peptide mapping of a tryptic digest and amino acid analyses of acid enzymic digests. The synthetic material is identical to natural human .gamma.-lipotropin when assayed against natural human .beta.-lipotropin for lipolytic activity in rabbit fat cells.