Detection and Quantitation of Bryostatin 1 and 2 inBugula Neritinaby Combined High-Peformance Liquid Chromatography and3H-Phorbol Dibutyrate Displacement
- 1 February 1990
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 13 (3) , 583-598
- https://doi.org/10.1080/01483919008051807
Abstract
An HPLC method has been developed to detect and quantitate bryostatins 1 and 2 from crude extracts of B. neritina. This, coupled with a 3H-phorbol dibutyrate binding assay and photodiode array-acquired uv spectra makes possible the evaluation of crude extracts in a rapid, sensitive and specific manner.Keywords
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