Effects of a pre‐incubation period on the photoinduced toxicity of polycyclic aromatic hydrocarbons to the luminescent bacterium Vibrio fischeri

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Abstract
Irradiation of polycyclic aromatic hydrocarbons (PAHs) in aqueous solution with simulated solar radiation (SSR; a light source with a visible light : UV‐A : UV‐B ratio similar to that of sunlight) can greatly enhance their toxicity. Two microbial toxicity tests with Vibrio fischeri were used to investigate the effect of composition of the growth medium and pre‐incubation on the photoinduced toxicity of PAHs. The assays were a short‐term test (15 min) and long‐term test (18 h). Both assays were carried out in SSR and darkness to examine for photoinduced toxicity of PAHs. For the short‐term toxicity assay, inhibition of bacterial luminescence was measured. For the long‐term toxicity assay, both inhibition of bacterial luminescence and inhibition of growth were recorded. To broaden this test, V. fischeri cells were pre‐incubated with PAHs in medium without a carbon source (minimal medium) for 8 h to facilitate assimilation and photooxidation of the contaminants, and to prevent bacterial growth at the outset of the assay. V. fischeri was more sensitive in minimal medium than in complex medium in both the short‐ and long‐term toxicity assays. Moreover, in the long‐term assay, SSR greatly increased toxicity, especially if there was a pre‐incubation period in minimal medium. This indicates that both assimilation and photooxidation of PAHs are important to their toxicity to V. fischeri. © 2001 John Wiley & Sons, Inc. Environ Toxicol 16: 277–286, 2001

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