Isolation of a High Molecular Mass Granulocyte Colony-Stimulating Factor from Bovine Lung Conditioned Medium
- 1 January 1982
- journal article
- research article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 363 (2) , 1493-1500
- https://doi.org/10.1515/bchm2.1982.363.2.1493
Abstract
A high-molecular mass type of granulocyte colony-stimulating factor (CSF-F) from bovine lung tissue produced by fibroblasts was isolated and partially purified by a 4-step procedure. Following ammonium sulfate fractionation, hydrophobic interaction, ion-exchange and affinity chromatography, CSF-F was > 200-fold enriched. CSF-F was a glycoprotein of .apprx. 68 kDa [kilodalton] with an isoelectric point of 4.0-5.2. It did not lose its biological activity after neuraminidase treatment. Gel-filtration behavior of desialo-CSF-F was not changed.This publication has 12 references indexed in Scilit:
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