Evaluation of PCR as a Means of Identification of Pasteurella pneumotropica.
- 1 January 1999
- journal article
- Published by Japanese Association for Laboratory Animal Science in Experimental Animals
- Vol. 48 (1) , 51-54
- https://doi.org/10.1538/expanim.48.51
Abstract
A polymerase chain reaction with new primers (new PCR) designed from Pasteurella pneumotropica 16S rDNA as an identification system for this organism was compared with the PCR reported by Wang et al. (Wang's PCR) by using 15 bacterial reference species and 70 clinical isolates with the conventional identification system. For the 15 reference strains, both PCRs were identical. For the 70 clinical isolates, the new PCR and Wang's PCR showed consistency with the conventional system in 62.9% (44/70) and 51.4% (36/70), respectively. Twenty-six isolates were inconsistent with the conventional system and the new PCR with respect to morphology and serology. These findings suggested that the new PCR was more sensitive than Wang's PCR, and the new PCR in combination with morphology and serology is useful for P. pneumotropica identification.Keywords
This publication has 3 references indexed in Scilit:
- Detection of Cilia-Associated Respiratory Bacillus in Experimentally and Naturally Infected Mice and Rats by the Polymerase Chain Reaction.Experimental Animals, 1995
- Phylogeny of 54 representative strains of species in the family Pasteurellaceae as determined by comparison of 16S rRNA sequencesJournal of Bacteriology, 1992
- An eye infection in laboratory mice associated with Pasteurella pneumotropicaLaboratory Animals, 1975