Mycotoxins - Their Biosynthesis in Fungi: Zearalenone Biosynthesis
- 1 November 1979
- journal article
- research article
- Published by Elsevier in Journal of Food Protection
- Vol. 42 (10) , 821-824
- https://doi.org/10.4315/0362-028x-42.10.821
Abstract
Zearalenone [6-(10-hydroxy-6-oxo-trans-1-undecenyl)-β-resorcylic acid lactone] is biosynthesized by Fusarium roseum by head-to-tail condensation of acetate units via the acetate-malonyl-CoA (polyketide) pathway. Incorporation and distribution of 1-[14C]acetate into zearalenone was demonstrated by degradation of the molecule into the aromatic ring, CO2, oxalic, succinic and glutaric acids. Incorporation and distribution of 1-[13C]acetate and 1,2-[13C]acetate was demonstrated by 13C-NMR and confirmed the conclusion reached by 14C-incorporation studies. CO2 is rapidly fixed by cultures of F. roseum, presumably by phosphoenolpyruvate carboxykinase and pyruvate carboxylase and incorporated into zearalenone. The wild-type isolates of F. roseum normally produce optimum amounts of zearalenone on a solid medium such as rice and at lower temperatures (10–14 C), although mutants are available that produce copious amounts in liquid medium. Some wild-type isolates also produce zearalenone optimally at 20–25 C.This publication has 0 references indexed in Scilit: