Protein and Antigenic Composition of Adult and Microfilarial Stages of Setaria Cervi

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Abstract
The protein and antigenic pattern of adult (female/male) and microfilarial stages of Setaria cervi, a bovine filarial parasite, was studied using certain immunochemical techniques. SDS-polyacrylamide gel electrophoretic analysis showed the presence of 35–40 protein bands in adults and 25–29 protein bands in microfilariae in molecular weight range of 10,000–200,000. Immunoelectrophoresis revealed the presence of 9–10 precipitin lines in adult and only 4 precipitin lines in microfilarial antigenic preparations. Crossed immuno-electrophoresis resolved these antigenic preparations further, and revealed the presence of 22–24 antigens in adults and 12–14 in microfilariae. These results demonstrate complex nature of somatic extracts of adult stage as compared to microfilariae and also reveal some qualitative and quantitative differences between these stages.